8VMP
Homing endonuclease I-PpoI-DNA complex:reaction at pH7.0 (K+ MES) with 500 uM Mg2+ for 10s
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-03-14 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9787 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 113.580, 113.580, 87.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.930 - 1.450 |
R-factor | 0.1796 |
Rwork | 0.179 |
R-free | 0.18510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.067 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.930 | 42.930 | 1.540 |
High resolution limit [Å] | 1.450 | 4.330 | 1.450 |
Rmerge | 0.080 | 0.048 | 0.617 |
Rmeas | 0.090 | 0.054 | 0.703 |
Number of reflections | 224615 | 8285 | 35634 |
<I/σ(I)> | 10.01 | ||
Completeness [%] | 99.2 | ||
Redundancy | 9 | ||
CC(1/2) | 0.997 | 0.996 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |