8VMO
Homing endonuclease I-PpoI-DNA complex:ground state at pH7.0 (K+ MES) with Na+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2023-03-14 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.9786 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 114.083, 114.083, 88.123 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.400 - 1.680 |
R-factor | 0.1703 |
Rwork | 0.169 |
R-free | 0.18910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.084 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
High resolution limit [Å] | 1.680 | 5.010 | 1.680 |
Rmerge | 0.088 | 0.044 | 0.845 |
Rmeas | 0.097 | 0.048 | 0.933 |
Number of reflections | 146560 | 5522 | 23406 |
<I/σ(I)> | 11.15 | ||
Completeness [%] | 99.8 | ||
Redundancy | 11 | ||
CC(1/2) | 0.998 | 0.998 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.2 M Sodium Malonate, PEG 3350, 0.1 M MES |