8VMB

The crystal structure of rhinovirus C15 RNA replication element sB-loop mutant in complex with Fab BL3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	I 21 21 21
Unit cell lengths	103.216, 121.847, 174.664
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	87.330 - 2.970
R-factor	0.2136
Rwork	0.210
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.150
Data reduction software	RAPD
Data scaling software	RAPD
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.930	3.150
High resolution limit [Å]	2.970	2.970
Number of reflections	23068	3652
<I/σ(I)>	19.2
Completeness [%]	99.8
Redundancy	6.8
CC(1/2)	1.000	0.643

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.2 M potassium chloride, 0.01 M calcium chloride dihydrate, 0.05 M sodium cacodylate trihydrate pH 6.0, 10% w/v polyethylene glycol 4000.