8VM8
The crystal structure of coxsackievirus B3 RNA replication element sD-loop mutant in complex with Fab BL3-6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-04-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 145.990, 80.370, 87.340 |
| Unit cell angles | 90.00, 111.17, 90.00 |
Refinement procedure
| Resolution | 38.310 - 1.540 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.764 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.310 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.049 | |
| Number of reflections | 138688 | 28390 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 4.2 | 4.1 |
| CC(1/2) | 0.999 | 0.348 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.2 M ammonium phosphate dibasic, 20% w/v polyethylene glycol 3350, pH 8.0. |
