8VLT
Crystal structure of glycan-targeting antibody 2526 Fab bound to Mannose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 19-ID |
| Synchrotron site | NSLS-II |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-28 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 |
| Unit cell lengths | 68.490, 68.490, 97.341 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.450 - 1.840 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.892 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.310 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.082 | 0.908 |
| Number of reflections | 43993 | 2672 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 75 mM HEPES pH 7.5, 10% PEG10K, 25% glycerol |
