8VLH

Crystal structure of Ash1L PHD-BAH domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-16
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5419
Spacegroup name	C 1 2 1
Unit cell lengths	173.110, 60.575, 53.588
Unit cell angles	90.00, 97.92, 90.00

Refinement procedure

Resolution	48.200 - 2.403
Rwork	0.230
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4L58 and 1W4S
RMSD bond length	0.002
RMSD bond angle	0.736
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MrBUMP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.063	0.319
Rmeas	0.077	0.429
Number of reflections	20953	1987
<I/σ(I)>	12.2	2.3
Completeness [%]	97.0
Redundancy	2.8
CC(1/2)	0.996	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Bis-Tris propane pH 10, 0.25 M strontium chloride, 0.01 M ammonium sulfate, and 25% PEG 8000