8VLF

Crystal structure of Ash1L PHD finger in complex with histone H3K4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.28
Spacegroup name	P 43
Unit cell lengths	49.800, 49.800, 46.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.070 - 1.340
R-factor	0.1568
Rwork	0.155
R-free	0.18840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7U7P
RMSD bond length	0.006
RMSD bond angle	0.956
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.070	1.390
High resolution limit [Å]	1.340	1.340
Rmerge	0.052	0.410
Number of reflections	49203	2524
<I/σ(I)>	12.5
Completeness [%]	99.9
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	1.6 M sodium citrate pH 6.5