8VKX
VX22 bound to GII.4 P domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-03-27 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.326, 148.980, 148.872 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.350 |
| Rwork | 0.245 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.410 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.198 | 0.628 |
| Rmeas | 0.230 | 0.735 |
| Rpim | 0.114 | 0.372 |
| Number of reflections | 18184 | 907 |
| <I/σ(I)> | 4.1 | 1 |
| Completeness [%] | 96.5 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.933 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1M LiSO4 15% PEG 8,000 |
