8VJZ

HLA-A*03:01 with WT KRAS-10mer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	2.1
Spacegroup name	P 6 2 2
Unit cell lengths	154.637, 154.637, 85.365
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.140 - 1.900
R-factor	0.185
Rwork	0.183
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.923
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.140	1.968
High resolution limit [Å]	1.900	1.900
Number of reflections	47684	4611
<I/σ(I)>	32.18	7.71
Completeness [%]	99.5
Redundancy	1.11
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1M ammonium citrate tribasic pH7, 12% PEG 3350