8VJX
Structure of Human Neurolysin in complex with bradykinin peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 143.826, 60.510, 96.558 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.560 - 2.890 |
R-factor | 0.2249 |
Rwork | 0.223 |
R-free | 0.25540 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond angle | 14.801 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.560 | 3.130 |
High resolution limit [Å] | 2.884 | 2.884 |
Rmerge | 0.278 | 1.894 |
Rmeas | 0.299 | 2.029 |
Rpim | 0.108 | 0.718 |
Total number of observations | 5746 | |
Number of reflections | 15148 | 758 |
<I/σ(I)> | 5.9 | 1.3 |
Completeness [%] | 77.0 | |
Redundancy | 7.3 | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 17.5 ~ 30 % polyethylene glycol 3,350 and 50 ~ 125 mM Bis-Tris HCl buffer, pH 6.5 |