8VJW
Structure of Human Neurolysin in complex with angiotensin I peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.078, 60.584, 147.759 |
| Unit cell angles | 90.00, 106.57, 90.00 |
Refinement procedure
| Resolution | 66.990 - 2.491 |
| R-factor | 0.2397 |
| Rwork | 0.237 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 10.045 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 141.622 | 2.844 |
| High resolution limit [Å] | 2.491 | 2.491 |
| Rmerge | 0.171 | 0.943 |
| Rmeas | 0.202 | 1.075 |
| Rpim | 0.106 | 0.512 |
| Total number of observations | 117633 | 7290 |
| Number of reflections | 33506 | 1675 |
| <I/σ(I)> | 5.6 | 1.5 |
| Completeness [%] | 55.3 | |
| Redundancy | 3.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 17.5 ~ 30 % polyethylene glycol 3,350 and 50 ~ 125 mM Bis-Tris HCl buffer, pH 6.5 |
