8VJW
Structure of Human Neurolysin in complex with angiotensin I peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-29 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 101.078, 60.584, 147.759 |
Unit cell angles | 90.00, 106.57, 90.00 |
Refinement procedure
Resolution | 66.990 - 2.491 |
R-factor | 0.2397 |
Rwork | 0.237 |
R-free | 0.28650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond angle | 10.045 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 141.622 | 2.844 |
High resolution limit [Å] | 2.491 | 2.491 |
Rmerge | 0.171 | 0.943 |
Rmeas | 0.202 | 1.075 |
Rpim | 0.106 | 0.512 |
Total number of observations | 117633 | 7290 |
Number of reflections | 33506 | 1675 |
<I/σ(I)> | 5.6 | 1.5 |
Completeness [%] | 55.3 | |
Redundancy | 3.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 17.5 ~ 30 % polyethylene glycol 3,350 and 50 ~ 125 mM Bis-Tris HCl buffer, pH 6.5 |