Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-03 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.827, 68.827, 79.251 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.640 - 1.580 |
| R-factor | 0.2041 |
| Rwork | 0.203 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.640 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.032 | 0.819 |
| Rpim | 0.011 | 0.294 |
| Number of reflections | 30191 | 1478 |
| <I/σ(I)> | 36.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.6 | |
| CC(1/2) | 1.000 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | HEPES-NaOH, Ammonium sulfate, PEG400, DTT |
