8VIR
Crystal structure of the N-terminal domain of fatty acid kinase A (FakA) from Staphylococcus aureus (Apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.048, 47.538, 93.232 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.350 - 1.900 |
| R-factor | 0.1763 |
| Rwork | 0.174 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.921 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3479: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.540 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 1.129 |
| Rmeas | 0.129 | 1.206 |
| Rpim | 0.046 | 0.421 |
| Total number of observations | 122554 | 7858 |
| Number of reflections | 15360 | 953 |
| <I/σ(I)> | 11 | 1.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 8 | 8.2 |
| CC(1/2) | 0.998 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Proplex D1: 25% (w/v) PEG 4000, 100 mM HEPES pH 7.5, 200 mM NaCl, Cryo: 80% well solution and 20% (v/v) PEG 200. |
