8VGZ

Influenza PA-N Endonuclease E23K Mutant (amino acids 52-74 truncation)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 62 2 2
Unit cell lengths	75.152, 75.152, 120.134
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	65.080 - 2.090
R-factor	0.2237
Rwork	0.218
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.669
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.080	2.170
High resolution limit [Å]	2.090	2.090
Rmerge	0.119
Number of reflections	19860	1198
<I/σ(I)>	9.2
Completeness [%]	99.0
Redundancy	18.5
CC(1/2)	0.999	0.173

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	30% PEG4000, 100 mM Tris, pH 8.35, 200-220 mM sodium acetate