8VGB

Crystal Structure of Guanine Nucleotide-Binding Protein Alpha Subunit (G Protein) from Oryza sativa in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	68.393, 68.419, 167.977
Unit cell angles	90.00, 89.91, 90.00

Refinement procedure

Resolution	48.370 - 2.990
R-factor	0.269
Rwork	0.266
R-free	0.30610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.091
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.040
High resolution limit [Å]	2.990	2.990
Rmeas	0.061	0.711
Rpim	0.031	0.362
Number of reflections	31650	1586
<I/σ(I)>	26.44	1.94
Completeness [%]	99.9
Redundancy	3.8
CC(1/2)		0.744

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	200 mM sodium acetate trihydrate (pH 8.5), 100 mM Tris (pH 8.5), 30% (w/v) PEG-4000