8VGA

Crystal Structure of Guanine Nucleotide-Binding Protein (G Protein) Alpha-1 Subunit from Selaginella moellendorffii in complex with GTP gamma S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	109.055, 61.468, 111.871
Unit cell angles	90.00, 115.23, 90.00

Refinement procedure

Resolution	42.622 - 2.576
R-factor	0.176964721126
Rwork	0.175
R-free	0.21639
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.913
Data reduction software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.610
High resolution limit [Å]	2.570	2.570
Rmeas	0.133	0.916
Rpim	0.069	0.474
Number of reflections	42607	2086
<I/σ(I)>	16.6	2.01
Completeness [%]	99.8
Redundancy	3.7
CC(1/2)		0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% (w/v) polyethylene glycol (PEG)-3350, 200 mM potassium citrate tribasic