8VFG

Binary DNA Polymerase Beta bound to DNA containing primer terminal FapydG base-paired with a dC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-13
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	54.380, 79.240, 54.880
Unit cell angles	90.00, 105.49, 90.00

Refinement procedure

Resolution	33.070 - 1.540
R-factor	0.2042
Rwork	0.201
R-free	0.23650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.570
High resolution limit [Å]	1.540	1.540
Number of reflections	58170	2370
<I/σ(I)>	27.1
Completeness [%]	97.6
Redundancy	3
CC(1/2)	0.999	0.379

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	14-22% PEG 3350 0.05 M Imidazole pH 8.0 0.35 M Sodium Acetate