8VF1
Crystal Structure of the Hendra Virus Attachment G glycoprotein (HeV-G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.822, 80.155, 95.136 |
| Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
| Resolution | 45.610 - 3.000 |
| R-factor | 0.2176 |
| Rwork | 0.215 |
| R-free | 0.26590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.627 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.610 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.176 | 0.636 |
| Rmeas | 0.211 | 0.808 |
| Rpim | 0.114 | 0.491 |
| Number of reflections | 16148 | 2554 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.976 | 0.634 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30-36% PEG 4000, 0.2 M MgCl2, and 0.1 M Tris pH 8.0-9.5 |
