8VEY
Crystal structure of PRMT5:MEP50 in complex with MTA and TNG908
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 102.310, 136.740, 177.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.330 - 2.440 |
| R-factor | 0.22468 |
| Rwork | 0.223 |
| R-free | 0.26136 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.176 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.230 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.085 | 1.134 |
| Rmeas | 0.089 | 1.178 |
| Rpim | 0.024 | 0.315 |
| Total number of observations | 647533 | 62519 |
| Number of reflections | 46656 | 4504 |
| <I/σ(I)> | 18.3 | 2.3 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.9 | 13.9 |
| CC(1/2) | 0.999 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 291 | 10% PEG 4000, 0.2M magnesium chloride, 0.1M sodium citrate (pH 6.0) |
