8VEV

Structure of a mouse IgG antibody antigen-binding fragment (Fab) targeting N6-methyladenosine (m6A), an RNA modification, m6A nucleoside ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-16
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.8731
Spacegroup name	P 21 21 21
Unit cell lengths	83.640, 128.377, 150.476
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.230 - 3.060
R-factor	0.225
Rwork	0.224
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.559
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.21rc1_5109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.230	43.230	3.180
High resolution limit [Å]	3.060	6.360	3.060
Rmerge	0.078	0.040	0.416
Rmeas	0.085	0.044	0.451
Rpim	0.033	0.017	0.172
Number of reflections	31244	3662	3399
<I/σ(I)>	16.8	34.8	4
Completeness [%]	99.9	99.7	100
Redundancy	6.5	6.1	6.8
CC(1/2)	0.998	0.998	0.963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	293	0.17 M Ammonium sulfate; 25,5 %(w/v) PEG 4000; 15 %(v/v) Glycerol, 0.2 mM m6A nucleoside