8VDI
Human PU.1 ETS-Domain (165-270) Bound to d(AATAAGAGGAAGTGGG) in Ternary Complex with DB2447
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-07-21 |
| Detector | MAR CCD 300 mm |
| Wavelength(s) | 1.0000000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.996, 60.583, 44.297 |
| Unit cell angles | 90.00, 116.64, 90.00 |
Refinement procedure
| Resolution | 33.140 - 1.930 |
| R-factor | 0.1683 |
| Rwork | 0.166 |
| R-free | 0.20180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.242 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.140 | 1.999 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.085 | 0.684 |
| Rmeas | 0.098 | 0.787 |
| Rpim | 0.048 | 0.386 |
| Number of reflections | 15325 | 1543 |
| <I/σ(I)> | 12.82 | 2.36 |
| Completeness [%] | 99.4 | 99.87 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.998 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | 100mM Sodium Acetate, pH=4.6, 2% PEG3350 |
