8VDB
Crystal structure of Bacillus subtilis FabHB, beta-ketoacyl carrier protein synthase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 91.964, 92.718, 93.998 |
| Unit cell angles | 111.66, 110.13, 105.45 |
Refinement procedure
| Resolution | 45.200 - 2.400 |
| R-factor | 0.2062 |
| Rwork | 0.206 |
| R-free | 0.22960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.789 |
| Data reduction software | DIALS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.140 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.156 | 0.874 |
| Rmeas | 0.182 | |
| Number of reflections | 88179 | 4618 |
| <I/σ(I)> | 4.5 | 1.8 |
| Completeness [%] | 93.2 | 94.1 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.983 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 20% MPD, 0.1 M HEPES, pH 6.5 |
