8VD9
Crystal structure of Bacillus subtilis FabHA, beta-ketoacyl carrier protein synthase III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.030, 61.030, 156.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.240 - 1.850 |
| R-factor | 0.2148 |
| Rwork | 0.212 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.653 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.030 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.074 | 0.584 |
| Rmeas | 0.077 | 0.609 |
| Number of reflections | 25612 | 1550 |
| <I/σ(I)> | 22.99 | 3.87 |
| Completeness [%] | 98.0 | |
| Redundancy | 16.77 | 12.34 |
| CC(1/2) | 0.999 | 0.884 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 50% PEG400, 0.2 mM NaCl, 0.1 M CHES, pH 9.5 |
