8VD9

Crystal structure of Bacillus subtilis FabHA, beta-ketoacyl carrier protein synthase III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	61.030, 61.030, 156.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.240 - 1.850
R-factor	0.2148
Rwork	0.212
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.653
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.030	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.074	0.584
Rmeas	0.077	0.609
Number of reflections	25612	1550
<I/σ(I)>	22.99	3.87
Completeness [%]	98.0
Redundancy	16.77	12.34
CC(1/2)	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	50% PEG400, 0.2 mM NaCl, 0.1 M CHES, pH 9.5