8VCM
Crystal structure of rMcL-1 in complex with galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 101 |
Detector technology | PIXEL |
Collection date | 2019-09-27 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.217, 41.094, 67.938 |
Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
Resolution | 34.690 - 2.210 |
R-factor | 0.1848 |
Rwork | 0.184 |
R-free | 0.20360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.154 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.21rc1_5156) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.119 | |
Number of reflections | 27697 | 2235 |
<I/σ(I)> | 27.2 | |
Completeness [%] | 99.5 | |
Redundancy | 5 | |
CC(1/2) | 0.861 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.2 M Na iodine, 0.1 M Bis-tris pronane pH 8.5, 20% PEG 3350 |