8VCC

Crystal structure of H19 influenza A virus hemagglutinin from A/lesser scaup/California/3087/2010

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979338
Spacegroup name	I 2 3
Unit cell lengths	203.262, 203.262, 203.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.909 - 2.383
R-factor	0.1875
Rwork	0.186
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.959
Data reduction software	XDS (Jan 10, 2022 (BUILT 20220110))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.600	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	55617	2794
<I/σ(I)>	15	1.4
Completeness [%]	99.9
Redundancy	17.3
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	0.1M HEPES pH 7.0, 15% PEG 20000