8VC9
Hybrid response regulator LvrB from Leptospira / phosphorylated REC form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.074, 102.327, 184.476 |
| Unit cell angles | 90.00, 92.49, 90.00 |
Refinement procedure
| Resolution | 49.270 - 2.650 |
| R-factor | 0.2124 |
| Rwork | 0.210 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rpim | 0.028 | 0.493 |
| Number of reflections | 94420 | 4642 |
| <I/σ(I)> | 14.4 | 1.4 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 4.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Tris-HCl, ammonium sulfate |
