8VBR

Structure of bovine anti-HIV Fab ElsE11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-15
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 21 21 21
Unit cell lengths	70.053, 70.187, 241.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.580 - 2.650
R-factor	0.3301
Rwork	0.247
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.568
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.580	2.720
High resolution limit [Å]	2.650	2.650
Rmerge	0.090	0.848
Rmeas	0.094	0.914
Rpim	0.027	0.320
Number of reflections	33457	1618
<I/σ(I)>	22	1.7
Completeness [%]	94.0	63.9
Redundancy	11.3	6.6
CC(1/2)	0.990	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	277	0.1M CHES, pH 9.5, 50% Peg200