8VBN

Structure of bovine anti-HIV Fab ElsE8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-15
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.97741
Spacegroup name	P 21 21 21
Unit cell lengths	59.217, 120.267, 150.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.950 - 1.830
R-factor	0.1962
Rwork	0.196
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.621
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.950	1.900
High resolution limit [Å]	1.830	1.830
Rmerge	0.116	1.730
Rmeas	0.120	1.830
Rpim	0.033	0.575
Number of reflections	95655	9259
<I/σ(I)>	15.9	1.3
Completeness [%]	99.8	98.2
Redundancy	12.8	9.8
CC(1/2)	0.999	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	20% Peg3350, 0.2M KCl