8VBN
Structure of bovine anti-HIV Fab ElsE8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-15 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.97741 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.217, 120.267, 150.302 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.950 - 1.830 |
R-factor | 0.1962 |
Rwork | 0.196 |
R-free | 0.21810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.621 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.950 | 1.900 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.116 | 1.730 |
Rmeas | 0.120 | 1.830 |
Rpim | 0.033 | 0.575 |
Number of reflections | 95655 | 9259 |
<I/σ(I)> | 15.9 | 1.3 |
Completeness [%] | 99.8 | 98.2 |
Redundancy | 12.8 | 9.8 |
CC(1/2) | 0.999 | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% Peg3350, 0.2M KCl |