8VBJ

Structure of bovine anti-HIV Fab ElsE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	69.959, 178.585, 105.706
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.480 - 1.900
R-factor	0.1938
Rwork	0.192
R-free	0.23110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.174
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.480	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.224	2.240
Rmeas	0.238	2.380
Rpim	0.079	0.776
Number of reflections	51953	5160
<I/σ(I)>	8.4	1.4
Completeness [%]	98.8	99.2
Redundancy	8.5	8.7
CC(1/2)	0.995	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1M Tris, pH 8.5, 1.5M ammonium sulfate, 12% glycerol