8VBJ
Structure of bovine anti-HIV Fab ElsE2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.959, 178.585, 105.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.480 - 1.900 |
| R-factor | 0.1938 |
| Rwork | 0.192 |
| R-free | 0.23110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.174 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.480 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.224 | 2.240 |
| Rmeas | 0.238 | 2.380 |
| Rpim | 0.079 | 0.776 |
| Number of reflections | 51953 | 5160 |
| <I/σ(I)> | 8.4 | 1.4 |
| Completeness [%] | 98.8 | 99.2 |
| Redundancy | 8.5 | 8.7 |
| CC(1/2) | 0.995 | 0.413 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris, pH 8.5, 1.5M ammonium sulfate, 12% glycerol |
