8VAX

Cadmium catalyzed primer extension product with 14mer primer and mixed phosphorothioate activated G monomer

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	99
Detector technology	PIXEL
Collection date	2023-03-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03579
Spacegroup name	P 3 2 1
Unit cell lengths	43.471, 43.471, 83.887
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.944 - 1.417
Rwork	0.175
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	2.868
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.417	1.420
Rmerge	0.095	0.286
Rmeas	0.101	0.350
Rpim	0.035	0.198
Number of reflections	16509	502
<I/σ(I)>	19.6	3.4
Completeness [%]	91.5	56.4
Redundancy	7.2	2.4
CC(1/2)	0.994	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	35% w/v 1,6-Hexanediol, 50 mM TRIS pH 8.5 75 mM Magnesium sulfate.