8VAX
Cadmium catalyzed primer extension product with 14mer primer and mixed phosphorothioate activated G monomer
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 2.0.1 |
| Synchrotron site | ALS |
| Beamline | 2.0.1 |
| Temperature [K] | 99 |
| Detector technology | PIXEL |
| Collection date | 2023-03-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03579 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 43.471, 43.471, 83.887 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.944 - 1.417 |
| Rwork | 0.175 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.868 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.440 |
| High resolution limit [Å] | 1.417 | 1.420 |
| Rmerge | 0.095 | 0.286 |
| Rmeas | 0.101 | 0.350 |
| Rpim | 0.035 | 0.198 |
| Number of reflections | 16509 | 502 |
| <I/σ(I)> | 19.6 | 3.4 |
| Completeness [%] | 91.5 | 56.4 |
| Redundancy | 7.2 | 2.4 |
| CC(1/2) | 0.994 | 0.834 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 35% w/v 1,6-Hexanediol, 50 mM TRIS pH 8.5 75 mM Magnesium sulfate. |
