8VAJ

Human Argonaute3 bound to cityRNA and target RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-17
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97934
Spacegroup name	P 2 21 21
Unit cell lengths	65.142, 138.126, 143.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.230 - 3.450
R-factor	0.2109
Rwork	0.206
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.688
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.230	3.573
High resolution limit [Å]	3.450	3.450
Rmeas	0.239	1.527
Number of reflections	17731	1724
<I/σ(I)>	7.1
Completeness [%]	99.5
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.8	293.15	0.14 M sodium citrate, 0.1 M Bis-Tris propane pH 8.8, and 18% (w/v) PEG 3350 supplemented with 4% (v/v) 1,3-Propanediol