8V9V

Yeh-like adhesin YhlD receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-03-29
Detector	RDI CMOS_8M
Wavelength(s)	0.9762
Spacegroup name	I 21 21 21
Unit cell lengths	28.302, 104.117, 131.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.820 - 2.290
R-factor	0.2256
Rwork	0.224
R-free	0.26310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.468
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.820	2.372
High resolution limit [Å]	2.290	2.290
Rmerge	0.066	0.687
Rmeas	0.093
Rpim	0.066
Number of reflections	9217	904
<I/σ(I)>	9.69	1.03
Completeness [%]	99.9
Redundancy	2
CC(1/2)	0.996	0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	95 mM sodium citrate, pH 5.6, 5% glycerol, 19% PEG4000, 19% isopropanol