8V9N

Human PU.1 ETS-Domain (165-270) Bound to d(AATAGAAGGAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-12-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000030
Spacegroup name	P 1 21 1
Unit cell lengths	42.928, 60.363, 44.707
Unit cell angles	90.00, 116.78, 90.00

Refinement procedure

Resolution	32.350 - 1.780
R-factor	0.172
Rwork	0.168
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.315
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.350	1.844
High resolution limit [Å]	1.780	1.780
Rmerge	0.060	0.519
Rmeas	0.069	0.597
Rpim	0.034	0.291
Number of reflections	19089	1900
<I/σ(I)>	17.53	3
Completeness [%]	97.3	98.8
Redundancy	4	4.1
CC(1/2)	0.999	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	100mM Sodium Acetate, pH=4.6, 2% PEG3350