8V9G
GES-5-meropenem complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.829, 80.581, 87.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.900 - 1.620 |
| R-factor | 0.1748 |
| Rwork | 0.173 |
| R-free | 0.21820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.117 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.200 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmeas | 0.106 | |
| Rpim | 0.035 | 0.428 |
| Number of reflections | 69480 | 3411 |
| <I/σ(I)> | 14.8 | 2.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 9.9 | |
| CC(1/2) | 0.999 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.4 | 277 | Crystallized spontaneously from 20 mM HEPES. Protein concentration 15 mg/ml |
