8V9A
GII.NA1 Loreto 1257 norovirus protruding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2023-04-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.704, 71.156, 78.386 |
| Unit cell angles | 90.00, 110.04, 90.00 |
Refinement procedure
| Resolution | 45.380 - 1.670 |
| R-factor | 0.1865 |
| Rwork | 0.185 |
| R-free | 0.21280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.035 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.380 | 1.690 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.135 | 1.309 |
| Rmeas | 0.153 | 1.481 |
| Rpim | 0.071 | 0.683 |
| Total number of observations | 344784 | 15533 |
| Number of reflections | 75438 | 3505 |
| <I/σ(I)> | 6.3 | 0.8 |
| Completeness [%] | 99.5 | |
| Redundancy | 4.6 | 4.4 |
| CC(1/2) | 0.995 | 0.453 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M Magnesium chloride hexahydrate 0.1 M Tris (Ph 7.0) 10% w/v PEG 8000 |
