8V94
De novo designed homo-oligomeric TM domain aITL_04927
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 22.059, 85.888, 10.759 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.370 - 1.240 |
| R-factor | 0.1638 |
| Rwork | 0.163 |
| R-free | 0.17490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.724 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.370 | 1.284 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.045 | 0.342 |
| Rmeas | 0.051 | 0.387 |
| Rpim | 0.023 | 0.177 |
| Number of reflections | 6170 | 574 |
| <I/σ(I)> | 21.2 | 2.21 |
| Completeness [%] | 97.0 | 93.94 |
| Redundancy | 4.7 | 4.4 |
| CC(1/2) | 0.999 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 0.1 M ADA pH 6.5, 0.1M NaCl, 0.1 M Li2SO4, 11.5 % PEG4000 |
