8V8R
Crystal Structure of Reactive Enamine Deaminase A (RidA) Homolog from an Opportunistic Pathogen, Streptococcus sanguinis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-29 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.509, 61.759, 75.725 |
| Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
| Resolution | 34.750 - 2.000 |
| R-factor | 0.2038 |
| Rwork | 0.201 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.464 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5144) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.115 | 0.869 |
| Rmeas | 0.125 | |
| Rpim | 0.046 | 0.619 |
| Number of reflections | 47778 | 2349 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.493 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5 10% v/v 2-Propanol 20% w/v Polyethylene Glycol 4000 |
