8V7P

Crystal structure of the truncated P1 pilin from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0236
Spacegroup name	P 1 21 1
Unit cell lengths	22.453, 54.853, 37.772
Unit cell angles	90.00, 96.14, 90.00

Refinement procedure

Resolution	37.555 - 1.300
Rwork	0.131
R-free	0.17380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.916
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.13)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.900	54.850	1.330
High resolution limit [Å]	0.940	6.500	1.300
Rmerge	0.141	0.070	0.498
Rmeas	0.158	0.083	0.615
Rpim	0.069	0.042	0.355
Number of reflections	22344	196	1309
<I/σ(I)>	14.2	28.7	3.9
Completeness [%]	99.5	99.4	99.2
Redundancy	4	3.9	2.7
CC(1/2)	0.988	0.998	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	35% PEG8000, 100 mM sodium cacodylate, 200 mM sodium chloride