8V7I

Human DNA polymerase eta-DNA-araC-ended primer ternary complex:reaction with 1 mM Mg2+ for 1800s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-09-21
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9786
Spacegroup name	P 61
Unit cell lengths	98.547, 98.547, 81.894
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.670 - 1.720
R-factor	0.2134
Rwork	0.212
R-free	0.23280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.175
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.672	42.672	1.820
High resolution limit [Å]	1.720	5.110	1.720
Rmerge	0.056	0.022	0.714
Rmeas	0.061	0.024	0.783
Number of reflections	47892	3567	15111
<I/σ(I)>	20.46
Completeness [%]	98.8
Redundancy	11.5
CC(1/2)	0.999	0.999	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES