8V7C

Human DNA polymerase eta-DNA-dT primer gemCTP insertion ternary complex at pH7.0 (K+ MES) with 1 Ca2+ ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-05
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9787
Spacegroup name	P 61
Unit cell lengths	98.854, 98.854, 82.122
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.810 - 1.790
R-factor	0.1799
Rwork	0.178
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.810	42.810	1.890
High resolution limit [Å]	1.790	5.320	1.790
Rmerge	0.065	0.034	0.721
Rmeas	0.072	0.037	0.795
Number of reflections	43207	3213	13676
<I/σ(I)>	15.29
Completeness [%]	99.8
Redundancy	11.3
CC(1/2)	0.999	0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES