8V7A

Human DNA polymerase eta-DNA-dT primer dCTP insertion ternary complex at pH7.0 (K+ MES) with 1 Ca2+ ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2021-04-15
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.9787
Spacegroup name	P 61
Unit cell lengths	98.193, 98.193, 81.746
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.520 - 1.950
R-factor	0.1734
Rwork	0.171
R-free	0.21820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.520	42.520	1.990
High resolution limit [Å]	1.880	5.590	1.880
Rmerge	0.063	0.022	0.986
Rmeas	0.070	0.025	1.102
Number of reflections	33210	2709	11228
<I/σ(I)>	15.89
Completeness [%]	98.8
Redundancy	11.3
CC(1/2)	0.999	1.000	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 2000MME, 0.1 M MES