8V61
UIC-1 mutant - UIC-1-L6I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-23 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 10.311, 28.251, 32.535 |
| Unit cell angles | 111.07, 90.26, 90.28 |
Refinement procedure
| Resolution | 15.850 - 1.130 |
| R-factor | 0.1862 |
| Rwork | 0.184 |
| R-free | 0.20790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.814 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.850 | 1.170 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.053 | 0.841 |
| Rmeas | 0.058 | 0.978 |
| Number of reflections | 12284 | 1104 |
| <I/σ(I)> | 16.88 | 1.28 |
| Completeness [%] | 95.2 | 86.61 |
| Redundancy | 4.9 | 3.6 |
| CC(1/2) | 0.999 | 0.817 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SLOW COOLING | 298 | water, acetonitrile |
