8V5Z

UIC-1 mutant - UIC-1-L6M

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.68880
Spacegroup name	P 21 21 21
Unit cell lengths	8.554, 16.942, 57.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	16.250 - 0.840
R-factor	0.1201
Rwork	0.118
R-free	0.13910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.922
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.250	0.870
High resolution limit [Å]	0.840	0.840
Rmerge	0.134	0.440
Rmeas	0.147	0.489
Number of reflections	6645	614
<I/σ(I)>	8.72	3.83
Completeness [%]	80.3
Redundancy	5.8
CC(1/2)	0.985	0.841

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SLOW COOLING		298	water, acetonitrile