8V5Q

Varicella Zoster Virus (VZV) glycoprotein E (gE) gI binding domain in complex with human Fab 1E3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	C 2 2 21
Unit cell lengths	44.621, 193.755, 179.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.720 - 1.900
R-factor	0.2266
Rwork	0.226
R-free	0.23310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.219
Data reduction software	AutoProcess
Data scaling software	AutoProcess
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.927	1.937
High resolution limit [Å]	1.900	1.900
Rmerge	0.184
Rmeas	0.191
Rpim	0.052
Number of reflections	61689	38641
<I/σ(I)>	8.9	0.9
Completeness [%]	99.8	96.5
Redundancy	13.7	13
CC(1/2)	0.996	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.6	295	0.1M citric acid, pH 3.6 ; 1.8M ammonium sulfate