8V59
UIC-1 mutant - UIC-1-B5I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-29 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.61992 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 54.012, 10.316, 32.278 |
| Unit cell angles | 90.00, 100.50, 90.00 |
Refinement procedure
| Resolution | 15.870 - 1.120 |
| R-factor | 0.1333 |
| Rwork | 0.131 |
| R-free | 0.15350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.708 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.870 | 1.160 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.168 | 0.344 |
| Rmeas | 0.187 | 0.381 |
| Rpim | 0.080 | 0.162 |
| Number of reflections | 6964 | 635 |
| <I/σ(I)> | 13.15 | 6.03 |
| Completeness [%] | 97.4 | 91.21 |
| Redundancy | 5.8 | 5.5 |
| CC(1/2) | 0.992 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SLOW COOLING | 298 | water, methanol |
