8V51

Crystal structure of a HLA-B*35:01-NP10 with D1 TCR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	I 1 2 1
Unit cell lengths	149.269, 46.155, 161.964
Unit cell angles	90.00, 101.40, 90.00

Refinement procedure

Resolution	39.500 - 2.100
R-factor	0.211963469969
Rwork	0.210
R-free	0.24425
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.624
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.037	0.280
Number of reflections	127751	12561
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG