8V44

N-terminal truncation of CRISPR-associated DinG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	85
Detector technology	IMAGE PLATE
Collection date	2023-11-03
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	67.040, 84.010, 97.619
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.760 - 2.900
R-factor	0.2306
Rwork	0.225
R-free	0.27720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.780
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.090	0.295
Number of reflections	926399	798
<I/σ(I)>	2.14
Completeness [%]	83.5
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	2:1.5 10.2 mg/mL CasDinG (50 mM Tris-HCl, 150 mM NaCl, 1.0 mM TCEP) : 0.1 M Tris-HCl pH 8.0, 22.5% PEG3350