8V34
Crystal structure of S. aureus TarK N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.885, 93.674, 136.760 |
| Unit cell angles | 90.00, 91.48, 90.00 |
Refinement procedure
| Resolution | 47.490 - 3.000 |
| R-factor | 0.2386 |
| Rwork | 0.236 |
| R-free | 0.28770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.567 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.490 | 3.107 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 45589 | 4546 |
| <I/σ(I)> | 11.96 | 1.68 |
| Completeness [%] | 99.7 | 99.74 |
| Redundancy | 3.4 | 3.6 |
| CC(1/2) | 0.998 | 0.676 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris pH 8.5, 100 mM NaCl, 75 mM MgCl2, 33% PEG 400 |
