8V2W
Crystal Structure of the ancestral triosephosphate isomerase reconstruction of the last opisthokont common ancestor obtained by Bayesian inference
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-30 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.525, 47.579, 64.308 |
| Unit cell angles | 90.00, 126.00, 90.00 |
Refinement procedure
| Resolution | 31.910 - 1.380 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.16990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.912 | 1.400 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.073 | 0.479 |
| Number of reflections | 46451 | 2219 |
| <I/σ(I)> | 27.3 | 2 |
| Completeness [%] | 98.5 | 96.8 |
| Redundancy | 3.2 | 2.1 |
| CC(1/2) | 0.910 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M BICINE pH 8.5, 15 % w/v PEG 1500 |
