8V2E
Crystal structure of B055 scaffold boost immunogen in complex with a mature 10E8 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-08-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.032, 84.941, 205.969 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.030 - 2.620 |
R-factor | 0.2306 |
Rwork | 0.228 |
R-free | 0.27630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.886 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (v1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.680 |
High resolution limit [Å] | 2.620 | 2.620 |
Number of reflections | 41203 | 2057 |
<I/σ(I)> | 20.6 | |
Completeness [%] | 99.7 | |
Redundancy | 11 | |
CC(1/2) | 0.985 | 0.502 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG 3350, calcium chloride |